It is intriguing how the mechanics of molecular motors is regulated to perform the mechanical work in living systems. Here we find that this rather precise regulationcan be due to that a “working” motor is arrested in a transitional state around certain motor force. Our analysis suggests that the motor force can be self-regulated through chemomechanical coupling, and motor force homeostasis is a built-in feature at the level of a single motor, which provides insights to understanding the coordinated function of multiple molecular motors existing in various physiological processes. http://appliedmechanics.asmedigitalcollection.asme.org/article.aspx?articleid=1713520